Research interests

  • structural biology
  • 3D cryo-electron microscopy
  • macromolecular machines
  • protein synthesis

Research Units in UniSysCat

Awards (selection)

2016 Member of the National Academy of Sciences Leopoldina
2014 Member of the European Molecular Biology Organization (EMBO)
2004‐2007 Young Investigator, European Molecular Biology Organization (EMBO)

Prof. Dr. Christian Spahn
Charité – Universitätsmedizin Berlin
Institut für Medizinische Physik und Biophysik
Charitéplatz 1
10117 Berlin 
+49 (0)30 450 524 131
+49 (0)30 450 524 952

Publications (selection)

Nikolay R., Hilal T., Schmidt S., Qin B., Schwefel D., Vieira-Vieira C.H., Mielke T., Bürger J., Loerke J., Amikura K., Flügel T., Ueda T., Selbach M., Deuerling E., Spahn C.M.T., Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. Mol Cell. 2021,

Kraushar M.L., Krupp F., Harnett D., Turko P., Ambrozkiewicz M.C., Sprink T., Imami K., Günnigmann M., Zinnall U., Vieira-Vieira C.H., Schaub T., Münster-Wandowski A., Bürger J., Borisova E., Yamamoto H., Rasin M.R., Ohler U., Beule D., Mielke T., Tarabykin V., Landthaler M., Kramer G., Vida I., Selbach M., Spahn C.M.T., Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit. Mol Cell. 2021, 81, 304-322

Krupp F., Said N., Huang Y.H., Loll B., Bürger J., Mielke T., Spahn C.M.T., Wahl M.C. (2019) Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor. Mol Cell. 2019, 74, 143-157.

Nikolay R., Hilal T., Qin B., Mielke T., Bürger J., Loerke J., Textoris-Taube K., Nierhaus K.H., Spahn C.M.T., Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution. Mol Cell. 2018, 70, 881-93

Behrmann E., Loerke J., Budkevich T.V., Yamamoto K., Schmidt A., Penczek P.A., Vos M.R., Bürger J., Mielke T., Scheerer P., Spahn C.M.T., Structural snapshots of actively translating human ribosomes. Cell. 2015, 161, 845-57

Yamamoto H., Unbehaun A., Loerke J., Behrmann E., Collier M., Bürger J., Scheerer P., Mielke T., Spahn C.M.T., Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV IRES RNA. Nat Struct Mol Biol. 2014, 21, 721-7

Budkevich T.V., Giesebrecht J., Behrmann E., Loerke J., Ramrath D., Mielke T., Ismer J., Hildebrand P., Tung C., Nierhaus K.H., Sanbonmatsu K.Y., Spahn C.M.T., Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement. Cell. 2014, 158, 121-31.

Ramrath D.J., Yamamoto H., Rother K., Wittek D., Pech M., Mielke T., Loerke J., Scheerer P., Ivanov P., Teraoka Y., Shpanchenko O., Nierhaus K.H., Spahn C.M.T., The complex of tmRNA-SmpB and EF-G on translocating ribosomes. Nature. 2012, 485, 526-9

Fritsch J., Scheerer P., Frielingsdorf S., Kroschinsky S., Friedrich B., Lenz O., Spahn C.M.T., The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre. Nature. 2011, 479, 249-52

Ratje A.H., Loerke J., Mikolajka A., Brunner M., Hildebrand P.W., Starosta A.L., Donhofer A., Connell S.R., Fucini P., Mielke T., Whitford P.C., Onuchic J.N., Yu Y., Sanbonmatsu K.Y., Hartmann R.K., Penczek P.A., Wilson D.N., Spahn C.M.T. Head swivel on the ribosome facilitates translocation by means of intra- subunit tRNA hybrid sites. Nature. 2010, 468, 713-6