Research interests

  • Biophysics of (cyano-)bacterial blue-light-responsive biological photoswitches with chromophore cofactors assembled in E. coli production strains
  • Fluorescence reporters for intracellular environment or metabolites
  • Fluorescence lifetime spectroscopy and analysis for biophysical or sensing applications
  • Energy transfer processes between chromophores between protein complexes in cyanobacterial photosynthesis
  • Spectroscopic reporter groups in photoreceptors and photoswitches

Research Units in UniSysCat

Awards (selection)

2007 Boris-Rajewski Price for achievements in biophysics

Prof. Dr. Thomas Friedrich
TU Berlin
Institute of Chemistry, PC 14
Straße des 17. Juni 135
10623 Berlin
+49 (0)30 314-24128
+49 (0)30 314-78600

Publications (selection)

F.-J. Schmitt, Z.Y. Campbell, M.V. Bui, A. Hüls, T. Tomo, M. Chen, E.G. Maksimov, S.I. Allakhverdiev, T. Friedrich, Photosynthesis supported by a chlorophyll f-dependent, entropy-driven uphill energy transfer in Halomicronema hongdechloris cells adapted to far-red light, Photosynthesis Research 2019, 139(1-3):185-201.

J. Märk, H. Dortay, A. Wagener, E. Zhang, J. Buchmann, C. Grötzinger, T. Friedrich, J. Laufer, Dual-wavelength 3D photoacoustic imaging of mammalian cells using a photoswitchable phytochrome reporter protein, Nature Communications Physics 2018, 1(1): 3.

D. Buhrke, N.N. Tavraz, D.M. Shcherbakova, L. Sauthof, M. Moldenhauer, F. Vélazquez Escobar, V.V. Verkhusha, P. Hildebrandt, T. Friedrich, Chromophore binding to two cysteines increases quantum yield of near-infrared fluorescent proteins, Scientific Reports 2018, 9:1866. 

N.N. Sluchanko, Y.B. Slonimsky, T. Friedrich, E.G. Maksomov, Topology of the OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria, Nature Communications  2018, 9(1):3869.

M. Moldenhauer, N. N. Sluchanko, D. Buhrke, D. V. Zlenko, N. N. Tavraz, F.-J. Schmitt, P. Hildebrandt, E. G. Maksimov, T. Friedrich, Assembly of photoactive Orange Carotenoid Protein from its domains unravels a carotenoid shuttle mechanism, Photosynthesis Research 2017, 133(1-3):327-341. 

E.G. Maksimov, N.N. Sluchanko, Y.B. Slonimskiy, E.A. Slutskaya, A.V. Stepanov, A.M. Argentova-Stevens, E.A. Shirshin, G.V. Tsoraev, K.E. Klementiev, O.V. Slatinskaya, E.P. Lukashev, T. Friedrich, V.Z. Paschenko, A.B. Rubin, The photocycle of orange carotenoid protein conceals distinct intermediates and asynchronous changes in the carotenoid and protein components, Scientific Reports 2017, 7:15548.

S. Wilkening, F.-J. Schmitt, M. Horch, I. Zebger, O. Lenz, T. Friedrich, Characterization of Frex as an NADH sensor for in vivo applications in the presence of NAD+ and at various pH valuesm, Photosynthesis Research 2017, 133(1-3): 305-315.

V. Tejwani, F.-J. Schmitt, S. Wilkening, I. Zebger, M. Horch, O. Lenz, T. Friedrich, Investigation of the NADH/NAD+ ratio in Ralstonia eutropha using the fluorescence reporter protein Peredox, BBA - Bioenergetics 2017, 1858 (1): 86–94.

E.G. Maksimov, N.N. Sluchanko, K.S. Mironov, E.A. Shirshin, K.E. Klementiev, G.V. Tsoraev, M. Moldenhauer, T. Friedrich, D.A. Los, S.I. Allakhverdiev,, V.Z. Paschenko, A.B. Rubin, Fluorescent labeling preserving OCP photoactivity reveals its reorganization during the photocycle, Biophysical Journal 2017, 112, 46–56.

D. Buhrke, F. Velazquez Escobar, L. Sauthof, S. Wilkening, N. Herder, N.N. Tavraz, M. Willoweit, A. Keidel, T. Utesch, M.-A. Mroginski, F.-J.Schmitt, P. Hildebrandt, T. Friedrich, The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP, Scientific Reports 2016, 6:28444.