Research interests

  • synthetic biology and genetic code expansion for bio-orthogonal chemistries
  • directed enzyme evolution, metabolic engineering (incl. strain engineering and evolution)
  • applications of an expanded genetic code to study protein stability, conformational dynamics, electrostatic features, multivalent binding proton and electron transfer, optical properties and folding
  • bio-expression of genetically encodable peptide-based drugs and complex bio-materials

Research Units in UniSysCat

Awards (selection)

2017 Publication Prize “Fluorochemistry” of AG Fluorine Chemistry of GDCh
2004 BioFuture Award

Prof. Dr. Nediljko Budisa
TU Berlin
Institute of Chemistry, Office L 1
Müller-Breslau-Straße 10
10623 Berlin
+49 (0)30 314-28821
+49 (0)30 314-28279

UniSysCat Publications

  • On the Role of a Conserved Tryptophan in the Chromophore Pocket of Cyanobacteriochrome, M. Blain-Hartung, G. Johannes von Sass, J. Plaickner, S. Katz, O. Tu Hoang, M. Andrea Mroginski, N. Esser, N. Budisa, K. T. Forest, P. Hildebrandt, Journal of Molecular Biology 2023, 168227–0, 10.1016/j.jmb.2023.168227
  • Orthogonal translation with 5‐cyanotryptophan as an infrared probe for local structural information, electrostatics, and hydrogen bonding, G. J. von Freiherr Sass, M. Blain‐Hartung, T. Baumann, K. T. Forest, P. Hildebrandt, N. Budisa, Protein Science 2023, 32, 10.1002/pro.4705
  • Local Electric Field Changes during the Photoconversion of the Bathy Phytochrome Agp2, A. Kraskov, J. von Sass, A. D. Nguyen, T. O. Hoang, D. Buhrke, S. Katz, N. Michael, J. Kozuch, I. Zebger, F. Siebert, P. Scheerer, M. A. Mroginski, N. Budisa, P. Hildebrandt, Biochemistry 2021, 60, 2967–2977, 10.1021/acs.biochem.1c00426
  • Xenobiology: A Journey towards Parallel Life Forms, N. Budisa, V. Kubyshkin, M. Schmidt, ChemBioChem 2020, 21, 2228–2231, 10.1002/cbic.202000141
  • Life as we don't (yet) know it, N. Budisa in Art as we don’t know it (Hrsg.: Berger, E.; Kasperi Mäki-Reinikka; Kira O’Reilly; Sederholm, H., ), Aalto University Publication Series, 2020, 0–0, ISBN: 9789526088228
  • Synthesis of New Aza‐ and Thia‐Crown Ether Based Amino Acids, T. Schneider, N. Brüssow, A. Yuvanc, N. Budisa, ChemistrySelect 2020, 5, 2854–2857, 10.1002/slct.202000122
  • Phage capsid nanoparticles with defined ligand arrangement block influenza virus entry, D. Lauster, S. Klenk, K. Ludwig, S. Nojoumi, S. Behren, L. Adam, M. Stadtmüller, S. Saenger, S. Zimmler, K. Hönzke, L. Yao, U. Hoffmann, M. Bardua, A. Hamann, M. Witzenrath, L. E. Sander, T. Wolff, A. C. Hocke, S. Hippenstiel, S. De Carlo, J. Neudecker, K. Osterrieder, N. Budisa, R. R. Netz, C. Böttcher, S. Liese, A. Herrmann, C. P. R. Hackenberger, Nature Nanotechnology 2020, 15, 373–379, 10.1038/s41565-020-0660-2
  • Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression, A. Perfumo, G. J. Freiherr von Sass, E. L. Nordmann, N. Budisa, D. Wagner, Frontiers in Microbiology 2020, 11, 10.3389/fmicb.2020.00881
  • Multiomics Analysis Provides Insight into the Laboratory Evolution of toward the Metabolic Usage of Fluorinated Indoles, F. Agostini, L. Sinn, D. Petras, C. J. Schipp, V. Kubyshkin, A. A. Berger, P. C. Dorrestein, J. Rappsilber, N. Budisa, B. Koksch, ACS Central Science 2020, 7, 81–92, 10.1021/acscentsci.0c00679
  • An Engineered Strain with Synthetic Metabolism for in‐Cell Production of Translationally Active Methionine Derivatives, C. J. Schipp, Y. Ma, A. Al‐Shameri, F. D'Alessio, P. Neubauer, R. Contestabile, N. Budisa, M. L. di Salvo, ChemBioChem 2020, 21, 3525–3538, 10.1002/cbic.202000257
  • Expanding the DOPA Universe with Genetically Encoded, Mussel‐Inspired Bioadhesives for Material Sciences and Medicine, N. Budisa, T. Schneider, ChemBioChem 2019, 20, 2163–2190, 10.1002/cbic.201900030
  • Anticipating alien cells with alternative genetic codes: away from the alanine world!, V. Kubyshkin, N. Budisa, Current Opinion in Biotechnology 2019, 60, 242–249, 10.1016/j.copbio.2019.05.006
  • Bilayer thickness determines the alignment of model polyproline helices in lipid membranes, V. Kubyshkin, S. L. Grage, A. S. Ulrich, N. Budisa, Physical Chemistry Chemical Physics 2019, 21, 22396–22408, 10.1039/c9cp02996f
  • Promotion of the collagen triple helix in a hydrophobic environment, V. Kubyshkin, N. Budisa, Organic & Biomolecular Chemistry 2019, 17, 2502–2507, 10.1039/c9ob00070d
  • The Alanine World Model for the Development of the Amino Acid Repertoire in Protein Biosynthesis, V. Kubyshkin, N. Budisa, International Journal of Molecular Sciences 2019, 20, 5507–0, 10.3390/ijms20215507
  • Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine, T. Baumann, M. Hauf, F. Richter, S. Albers, A. Möglich, Z. Ignatova, N. Budisa, International Journal of Molecular Sciences 2019, 20, 2343–0, 10.3390/ijms20092343
  • In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation, S. Nojoumi, Y. Ma, S. Schwagerus, C. P. R. Hackenberger, N. Budisa, International Journal of Molecular Sciences 2019, 20, 2299–0, 10.3390/ijms20092299
  • Site‐Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater, T. Baumann, M. Hauf, F. Schildhauer, K. B. Eberl, P. M. Durkin, E. Deniz, J. G. Löffler, C. G. Acevedo‐Rocha, J. Jaric, B. M. Martins, H. Dobbek, J. Bredenbeck, N. Budisa, Angewandte Chemie International Edition 2019, 58, 2899–2903, 10.1002/anie.201812995

Publications (selection)

T. Baumann, M. Hauf, F. Schildhauer, K.B. Eberl, P.M. Durkin, E. Deniz, J.G. Löffler, C.G. Acevedo-Rocha, J. Jaric, B.M. Martins, H. Dobbek, J. Bredenbeck, N. Budisa, Observation of Site-Resolved Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater, Angewandte Chemie International Edition 2019, In press (doi: 10.1002/anie.201812995).

V. Kubyshkin, S.L. Grage, J. Bürck, A.S. Ulrich, N. Budisa, Transmembrane Polyproline Helix, The Journal of Physical Chemistry Letters 2018, 9(9), 2170-2174

M. Bartholomae, T. Baumann, J.H. Nickling, D. Peterhoff, R. Wagner, N. Budisa, O.P. Kuipers, Expanding the genetic code of Lactococcus lactis and Escherichia coli to incorporate non-canonical amino acids for production of modified lantibiotics, Frontiers in Microbiology 2018, 9, 657

P. Mykhailiuk, V. Kubyshkin, T. Bach, N. Budisa, A peptidyl-prolyl model study: how does the electronic effect influence the amide bond conformation?, Journal of Organic Chemistry 2017, 82, 8831–8841.

F. Agostini, J-S. Voller, B. Koksch, C.G. Acevedo-Rocha, V. Kubyshkin, N. Budisa, Xenobiology meets enzymology: exploring the potential of unnatural building blocks in biocatalysis, Angewandte Chemie International Edition 2017, 56, 9680–9703.

J. Völler, M. Dulic, U. Gerling-Driessen, H. Biava, T. Baumann, N. Budisa, I. Gruic, B. Koksch, Discovery and investigation of natural editing function against artificial amino acids in protein translation, ACS Central Science 2017, 3, 73–80.

M.P. Exner, T. Kuenzl, S. Schwagerus, T. To, Z. Ouyang, M.G. Hoesl, M.C. Lensen, C. P. R. Hackenberger, S. Panke, N. Budisa, Design of an S-allylcysteine in situ production and incorporation system based on a novel pyrrolysyl-tRNA synthetase variant, ChemBioChem 2017, 18, 85–90.

M. Hauf, F. Richter, T. Schneider, T. Faidt, B. M. Martins, T. Baumann, P. Durkin, H. Dobbek, K. Jacobs, A. Moeglich, N. Budisa, Photoactivatable mussel-based underwater adhesive proteins by an expanded genetic code, ChemBioChem 2017, 18, 1819.

A. Kuthning, P. Durkin, S. Oehm, M. Hoesl, N. Budisa, R. Süssmuth, Towards biocontained cell factories: an evolutionarily adapted escherichia coli strain produces a new-to-nature bioactive lantibiotic containing thienopyrrole-alanine, Scientific Reports 2016, 6, 33447.

M.G. Hoesl, S. Oehm, P. Durkin, E. Darmon, L. Peil, H-R. Aerni, J. Rappsilber, J. Rinehart, D. Leach, D. Söll, N. Budisa, Chemical evolution of a bacterial proteome, Angewandte Chemie International Edition 2015, 54, 10030–10034.

L. K. Doerfel, I. Wohlgemuth, V. Kubyshkin, A. L. Starosta, D. N. Wilson, N. Budisa, M. V. Rodnina, Entropic contribution of elongation factor P to proline positioning at the catalytic center of the ribosome, Journal of the American Chemical Society 2015, 137, 12997–13006.

S. Lepthien, L. Merkel, N. Budisa, In vivo double and triple labeling of proteins using synthetic amino acids, Angewandte Chemie International Edition 2010, 49, 5446–5450.

S. Lepthien, H. G. Hoesl, L. Merkel, N. Budisa, Azatryptophans endow proteins with intrinsic blue fluorescence, Proceedings of the National Academy of Sciences of the United States of America 2008, 49, 5446–5450.


N. Budisa et al, Photoactivatable, mussel-based bio-adhesives for treatment of bone fractures and wound healing, 2017, 16057/TUB.