Research interests

  • structure and mechanisms of complex metalloenzymes
  • enzymes in the bacterial one-carbon metabolism
  • ATPase-dependent electron transfer reactions
  • Ni-dependent enzymes and their maturation
  • complex iron-sulfur dependent enzymes

Research Units in UniSysCat

Awards (selection)

2021 Member of the Berlin-Brandenburg Academy of Sciences and Humanities
2011 Steinhofer Lecture Award, U Freiburg
2004 Junior Research Award of the U Bayreuth
2002 Junior Research Award of the Max Planck Institute of Biochemistry, Martinsried
2002 Krka Award of pharmaceutical company Krka, Novo Mesto (Slovenia)

Prof. Dr. Holger Dobbek 
HU Berlin
Department of Biology
Philippstraße 13
10115 Berlin
+49 (0)30 2093-6369
+49 (0)30 2093-6447

UniSysCat Publications

  • Discovery of the Lanthipeptide Curvocidin and Structural Insights into its Trifunctional Synthetase CuvL, A. Sigurdsson, B. M. Martins, S. A. Düttmann, M. Jasyk, B. Dimos‐Röhl, F. Schöpf, M. Gemander, C. H. Knittel, R. Schnegotzki, B. Schmid, S. Kosol, L. Pommerening, M. Gonzáles‐Viegaz, M. Seidel, M. Hügelland, S. Leimkühler, H. Dobbek, A. Mainz, R. D. Süssmuth, Angewandte Chemie International Edition 2023, 10.1002/anie.202302490
  • Determining the oxidation state of elements by X-ray crystallography, F. Lennartz, J. H. Jeoung, S. Ruenger, H. Dobbek, M. S. Weiss, Acta Crystallographica Section D Structural Biology 2022, 78, 238–247, 10.1107/S2059798321013048
  • A Morphing [4Fe‐3S‐nO]‐Cluster within a Carbon Monoxide Dehydrogenase Scaffold, J. Jeoung, J. Fesseler, L. Domnik, F. Klemke, M. Sinnreich, C. Teutloff, H. Dobbek, Angewandte Chemie International Edition 2022, 10.1002/anie.202117000
  • Structural basis for coupled ATP-driven electron transfer in the double-cubane cluster protein, J. H. Jeoung, S. Nicklisch, H. Dobbek, Proceedings of the National Academy of Sciences 2022, 119, 10.1073/pnas.2203576119
  • On the Kinetics of CO Reduction by Ni, Fe-CO Dehydrogenases, J. Ruickoldt, Y. Basak, L. Domnik, J. H. Jeoung, H. Dobbek, ACS Catalysis 2022, 13131–13142, 10.1021/acscatal.2c02221
  • Substrate Activation at the Ni,Fe Cluster of CO Dehydrogenases: The Influence of the Protein Matrix, Y. Basak, J. H. Jeoung, L. Domnik, J. Ruickoldt, H. Dobbek, ACS Catalysis 2022, 12711–12719, 10.1021/acscatal.2c02922
  • Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition, R. Hussein, M. Ibrahim, A. Bhowmick, P. S. Simon, R. Chatterjee, L. Lassalle, M. Doyle, I. Bogacz, I. S. Kim, M. H. Cheah, S. Gul, C. de Lichtenberg, P. Chernev, C. C. Pham, I. D. Young, S. Carbajo, F. D. Fuller, R. Alonso-Mori, A. Batyuk, K. D. Sutherlin, A. S. Brewster, R. Bolotovsky, D. Mendez, J. M. Holton, N. W. Moriarty, P. D. Adams, U. Bergmann, N. K. Sauter, H. Dobbek, J. Messinger, A. Zouni, J. Kern, V. K. Yachandra, J. Yano, Nature Communications 2021, 12, 10.1038/s41467-021-26781-z
  • ATP Binding and a Second Reduction Enables a Conformationally Gated Uphill Electron Transfer, F. Neumann, H. Dobbek, ACS Catalysis 2021, 11, 8565–8575, 10.1021/acscatal.1c01038
  • Bimetallic Mn, Fe, Co, and Ni Sites in a Four-Helix Bundle Protein: Metal Binding, Structure, and Peroxide Activation, J. H. Jeoung, S. Rünger, M. Haumann, B. Neumann, F. Klemke, V. Davis, A. Fischer, H. Dau, U. Wollenberger, H. Dobbek, Inorganic Chemistry 2021, 60, 17498–17508, 10.1021/acs.inorgchem.1c01919
  • Untangling the sequence of events during the S → S transition in photosystem II and implications for the water oxidation mechanism, M. Ibrahim, T. Fransson, R. Chatterjee, M. H. Cheah, R. Hussein, L. Lassalle, K. D. Sutherlin, I. D. Young, F. D. Fuller, S. Gul, I. S. Kim, P. S. Simon, C. de Lichtenberg, P. Chernev, I. Bogacz, C. C. Pham, A. M. Orville, N. Saichek, T. Northen, A. Batyuk, S. Carbajo, R. Alonso-Mori, K. Tono, S. Owada, A. Bhowmick, R. Bolotovsky, D. Mendez, N. W. Moriarty, J. M. Holton, H. Dobbek, A. S. Brewster, P. D. Adams, N. K. Sauter, U. Bergmann, A. Zouni, J. Messinger, J. Kern, V. K. Yachandra, J. Yano, Proceedings of the National Academy of Sciences 2020, 117, 12624–12635, 10.1073/pnas.2000529117
  • pH‐Dependent Protonation of Surface Carboxylate Groups in PsbO Enables Local Buffering and Triggers Structural Changes, L. Gerland, D. Friedrich, L. Hopf, E. J. Donovan, A. Wallmann, N. Erdmann, A. Diehl, M. Bommer, K. Buzar, M. Ibrahim, P. Schmieder, H. Dobbek, A. Zouni, A. Bondar, H. Dau, H. Oschkinat, ChemBioChem 2020, 21, 1597–1604, 10.1002/cbic.201900739
  • The two CO-dehydrogenases of Thermococcus sp. AM4, M. Benvenuti, M. Meneghello, C. Guendon, A. Jacq-Bailly, J. H. Jeoung, H. Dobbek, C. Léger, V. Fourmond, S. Dementin, Biochimica et Biophysica Acta (BBA) - Bioenergetics 2020, 1861, 148188–0, 10.1016/j.bbabio.2020.148188
  • Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology, J. Jeoung, B. M. Martins, H. Dobbek, ChemBioChem 2020, 21, 1710–1716, 10.1002/cbic.202000016
  • Investigating the Structure and Dynamics of Apo‐Photosystem II, R. Han, K. Rempfer, M. Zhang, H. Dobbek, A. Zouni, H. Dau, S. Luber, ChemCatChem 2019, 11, 4072–4080, 10.1002/cctc.201900351
  • Site‐Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater, T. Baumann, M. Hauf, F. Schildhauer, K. B. Eberl, P. M. Durkin, E. Deniz, J. G. Löffler, C. G. Acevedo‐Rocha, J. Jaric, B. M. Martins, H. Dobbek, J. Bredenbeck, N. Budisa, Angewandte Chemie International Edition 2019, 58, 2899–2903, 10.1002/anie.201812995
  • X‐ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases, Y. Ilina, C. Lorent, S. Katz, J. Jeoung, S. Shima, M. Horch, I. Zebger, H. Dobbek, Angewandte Chemie International Edition 2019, 58, 18710–18714, 10.1002/anie.201908258

Publications (selection)

M. Zhang, M. Bommer, R. Chatterjee, R. Hussein, J. Yano, H. Dau, J. Kern, H. Dobbek, A. Zouni, Structural Insights into the light-driven auto-assembly process of the water oxidizing Mn4CaO5-cluster in photosystem II, eLife 2017, 6, e26933.

T. Werther, S. Wahlefeld, J. Salewski, U. Kuhlmann, I. Zebger, P. Hildebrandt, H. Dobbek, Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase, Nature Communications 2017, 8, 1–8.

L. Domnik, M. Merrouch, S. Goetzl, J. H. Jeoung, C. Leger, S. Dementin, V. Fourmond, H. Dobbek, CODH-IV: a high-efficiency CO-scavenging CO dehydrogenase with resistance to O2, Angewandte Chemie International Edition 2017, 56, 15466–15469.

J. H. Jeoung, D. Nianios, S. Fetzner, H. Dobbek, Quercetin 2,4-dioxygenase activates dioxygen in a side-on O2-Ni complex, Angewandte Chemie International Edition 2016, 55, 3281–3284.

I. D. Young, M. Ibrahim, R. Chatterjee, S. Gul, F. D. Fuller, S. Koroidov, A. S. Brewster, R. Tran, R. Alonso-Mori, T. Kroll, T. Michels-Clark, H. Laksmono, R. G. Sierra, C. A. Stan, R. Hussein, M. Zhang, L. Douthit, M. Kubin, C. de Lichtenberg, L. Vo Pham, H. Nilsson, M. H. Cheah, D. Shevela, C. Saracini, M. A. Bean, I. Seuffert, D. Sokaras, T. C. Weng, E. Pastor, C. Weninger, T. Fransson, L. Lassalle, P. Bräuer, P. Aller, P. T. Docker, B. Andi, A. M. Orville, J. M. Glownia, S. Nelson, M. Sikorski, D. Zhu, M. S. Hunter, T. J. Lane, A. Aquila, J. E. Koglin, J. Robinson, M. Liang, S. Boutet, A. Y. Lyubimov, M. Uervirojnangkoorn, N. W. Moriarty, D. Liebschner, P. V. Afonine, D. G. Waterman, G. Evans, P. Wernet, H. Dobbek, W. I. Weis, A. T. Brunger, P. H. Zwart, P. D. Adams, A. Zouni, J. Messinger, U. Bergmann, N. K. Sauter, J. Kern, V. K. Yachandra, J. Yano, Structure of photosystem II and substrate binding at room temperature, Nature 2016, 540, 453–457.

J. Fesseler, J. H. Jeoung, H. Dobbek, How the [NiFe4S4] cluster of CO dehydrogenase activates CO2 and NCO, Angewandte Chemie International Edition 2015, 54, 8560–8564.

S. E. Hennig, S. Goetzl, J. H. Jeoung, M. Bommer, F. Lendzian, P. Hildebrandt, H. Dobbek, ATP-induced electron transfer by redox-selective partner recognition, Nature Communications 2014, 5, 4626.

M. Bommer, C. Kunze, J. Fesseler, T. Schubert, G. Diekert, H. Dobbek, Structural basis for organohalide respiration, Science 2014, 346, 455–458.

J. H. Jeoung, M. Bommer, T. Y. Lin, H. Dobbek, Visualizing the substrate-, superoxo-, alkylperoxo-, and product-bound states at the nonheme Fe(II) site of homogentisate dioxygenase, Proceedings of the National Academy of Sciences of the United States of America 2013, 110, 12625–12630.

J. H. Jeoung, H. Dobbek, Carbon dioxide activation at the Ni,Fe-cluster of anaerobic carbon monoxide dehydrogenase, Science 2007, 318, 1461–1464.