Unit C: Coupling of chemo- and biocatalysis

Our Challenge

Concepts rather than reactions of chemical and biological catalysis will be combined to design and develop semi-artificial enzymes and hybrid devices with new catalytic properties.

Our Approach

The Generation of semi-artificial enzymes will be approached from both the biological and chemical direction, either by engineering naturally occurring small enzymes or by inserting synthetic cofactors (molecular metal complexes) into a tailored proteinaceous environment.

Hybrid biocatalysts and enzyme systems (i.e., whole living cells) will be studied either immobilized on or encapsulated in smart support materials, e.g., via molecular imprinting. Material design with regard to biocompatibility, conductivity and optical properties will be performed in close collaboration with Unit A and Unit D.

The central goal of this Research Unit is to develop new catalytic systems, assembled from well-understood native enzymes (Unit B). These new systems could be genetically modified to accommodate synthetic cofactors with new functionalities (Unit A). One of the target systems will be photosystem II lacking the water-oxidizing Mn4CaO5 cluster. The corresponding empty binding site will be refilled with alternative divalent metals to assemble multinuclear metal complexes whose catalytic competency will be evaluated. 

In a long-term perspective, this may contribute to the establishment of a predictable systemic model for the design of chemzymes – biocatalysts that carry chemically modified catalytic centers and show novel reactivities.

Team of Unit C

Robert Bittl
EPR spectroscopy

Nediljko Budisa
Protein modification by non-natural amino acids

Thomas Braun
Metal organic chemistry, late-metal catalysis

Holger Dau
X-ray spectroscopy

Holger Dobbek
Biochemistry, X-ray crystallography

Matthias Driess
Organometallic chemistry, ligand design

Thomas Friedrich
Fluorescence spectroscopy (insitu)

Peter Hildebrandt
Raman spectroscopy (surface-sensitive, in situ)

Marius Horch
Ultrafast, multidimensional and in vivo vibrational spectroscopy

Silke Leimkühler
Molecular enzymology

Oliver Lenz
Biochemistry, enzyme engineering

Christian Limberg
Ligand design, small molecule activation

Maria Andrea Mroginski
Theory (QM/MM, MD)

Ariane Nunes Alves
Molecular modeling and simulation of ligand-protein interactions

Martin Oestreich
Organic synthesis, ligand design, FLP


Kallol Ray
Lewis acid cooperation, mechanism

Patrick Scheerer
X-ray crystallography, XFEL


Frieder Scheller
enzyme electrochemistry


Christian Teutloff
EPR and hyperfine spectroscopy


Arne Thomas
Materials synthesis

Ulla Wollenberger
Protein electrochemistry

Ingo Zebger
IR spectroscopy (surface-sensitive, in situ)

Athina Zouni
Assembly/disassembly of PSII, XFEL

Contact Unit C

Dr. Oliver Lenz
TU Berlin
Institute of Chemistry, Office PC 14
Straße des 17. Juni 135
10623 Berlin
+49 (0)30 314-25650

Prof. Dr. Christian Limberg
HU Berlin
Department of Chemistry
Brook-Taylor-Straße 2
12489 Berlin
+49 (0)30 2093-7382