Prof. Dr. Nediljko Budisa

Research interests

  • synthetic biology and genetic code expansion for bio-orthogonal chemistries
  • directed enzyme evolution, metabolic engineering (incl. strain engineering and evolution)
  • applications of an expanded genetic code to study protein stability, conformational dynamics, electrostatic features, multivalent binding proton and electron transfer, optical properties and folding
  • bio-expression of genetically encodable peptide-based drugs and complex bio-materials

Awards (selection)

2017 Publication Prize “Fluorochemistry” of AG Fluorine Chemistry of GDCh
2004 BioFuture Award

Prof. Dr. Nediljko Budisa
TU Berlin
Institute of Chemistry, Office L 1
Müller-Breslau-Straße 10
10623 Berlin
+49 (0)30 314-28821
+49 (0)30 314-28279

Publications (selection)

T. Baumann, M. Hauf, F. Schildhauer, K.B. Eberl, P.M. Durkin, E. Deniz, J.G. Löffler, C.G. Acevedo-Rocha, J. Jaric, B.M. Martins, H. Dobbek, J. Bredenbeck, N. Budisa, Observation of Site-Resolved Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater, Angewandte Chemie International Edition2019, In press (doi: 10.1002/anie.201812995).

V. Kubyshkin, S.L. Grage, J. Bürck, A.S. Ulrich, N. Budisa, Transmembrane Polyproline Helix, The Journal of Physical Chemistry Letters2018, 9(9), 2170-2174

M. Bartholomae, T. Baumann, J.H. Nickling, D. Peterhoff, R. Wagner, N. Budisa, O.P. Kuipers, Expanding the genetic code of Lactococcus lactis and Escherichia coli to incorporate non-canonical amino acids for production of modified lantibiotics, Frontiers in Microbiology2018, 9, 657

P. Mykhailiuk, V. Kubyshkin, T. Bach, N. Budisa, A peptidyl-prolyl model study: how does the electronic effect influence the amide bond conformation?, Journal of Organic Chemistry 2017, 82, 8831–8841.

F. Agostini, J-S. Voller, B. Koksch, C.G. Acevedo-Rocha, V. Kubyshkin, N. Budisa, Xenobiology meets enzymology: exploring the potential of unnatural building blocks in biocatalysis, Angewandte Chemie International Edition 2017, 56, 9680–9703.

J. Völler, M. Dulic, U. Gerling-Driessen, H. Biava, T. Baumann, N. Budisa, I. Gruic, B. Koksch, Discovery and investigation of natural editing function against artificial amino acids in protein translation, ACS Central Science 2017, 3, 73–80.

M.P. Exner, T. Kuenzl, S. Schwagerus, T. To, Z. Ouyang, M.G. Hoesl, M.C. Lensen, C. P. R. Hackenberger, S. Panke, N. Budisa, Design of an S-allylcysteine in situ production and incorporation system based on a novel pyrrolysyl-tRNA synthetase variant, ChemBioChem 2017, 18, 85–90.

M. Hauf, F. Richter, T. Schneider, T. Faidt, B. M. Martins, T. Baumann, P. Durkin, H. Dobbek, K. Jacobs, A. Moeglich, N. Budisa, Photoactivatable mussel-based underwater adhesive proteins by an expanded genetic code, ChemBioChem 2017, 18, 1819.

A. Kuthning, P. Durkin, S. Oehm, M. Hoesl, N. Budisa, R. Süssmuth, Towards biocontained cell factories: an evolutionarily adapted escherichia coli strain produces a new-to-nature bioactive lantibiotic containing thienopyrrole-alanine, Scientific Reports 2016, 6, 33447.

M.G. Hoesl, S. Oehm, P. Durkin, E. Darmon, L. Peil, H-R. Aerni, J. Rappsilber, J. Rinehart, D. Leach, D. Söll, N. Budisa, Chemical evolution of a bacterial proteome, Angewandte Chemie International Edition 2015, 54, 10030–10034.

L. K. Doerfel, I. Wohlgemuth, V. Kubyshkin, A. L. Starosta, D. N. Wilson, N. Budisa, M. V. Rodnina, Entropic contribution of elongation factor P to proline positioning at the catalytic center of the ribosome, Journal of the American Chemical Society 2015, 137, 12997–13006.

S. Lepthien, L. Merkel, N. Budisa, In vivo double and triple labeling of proteins using synthetic amino acids, Angewandte Chemie International Edition 2010, 49, 5446–5450.

S. Lepthien, H. G. Hoesl, L. Merkel, N. Budisa, Azatryptophans endow proteins with intrinsic blue fluorescence, Proceedings of the National Academy of Sciences of the United States of America 2008, 49, 5446–5450.


N. Budisa et al, Photoactivatable, mussel-based bio-adhesives for treatment of bone fractures and wound healing, 2017, 16057/TUB.